Productos - Biología molecular - Ácidos nucleicos - Microbiomics

ZymoBIOMICS DNA Miniprep Kit

Ref. D4304

Los kits de ADN de ZymoBIOMICS son kits de purificación de ADN microbiano diseñados para purificar el ADN de una variedad de entradas de muestra que están inmediatamente listas para análisis de microbiomas o metagenomas.

Características de Producto

Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
Certified Low Bioburden: Boost your detection limit for low abundance microbes.

Descripción de Producto

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyzes. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative / positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

Especificaciones Técnicas

Applicable For	All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume	≥ 50 µl DNA Elution Buffer or DNase free water
Equipment	Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume	Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x10⁹ bacterial, 2x10⁸ yeast, or 2x10⁷ mammalian cells)
Purity	High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source	Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample Storage	Eluted DNA should be stored at ≤ -20°C.
Size Range	Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type	Total DNA
Yield	Up to 25 µg total DNA can be eluted into ≥50 µl

Código de Producto

Cat #	Name	Size
D4300	ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes)	50 Preps
D4300T	ZymoBIOMICS DNA Miniprep Kit	5 Preps
D4304	ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix)	50 Preps