Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
Elution Volume |
≥ 10 µl ZymoBIOMICS DNase/RNase Free Water |
Equipment |
Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). |
Processing Volume |
Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
Purity |
High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 |
Sample Source |
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
Sample Storage |
Eluted DNA should be stored at ≤ -20°C. |
Size Range |
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
Type |
Total DNA |
Yield |
Up to 5 µg total DNA can be eluted into ≥ 10 µl |