HIGHLIGHTS
- Simple workflow: Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries in as little as 2 hours of hands-on time.
- Low input: The only RRBS kit that produces NGS libraries from ≥ 10 ng of genomic DNA.
- Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.
DESCRIPTION
Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS).
This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome.
This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.
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TECHNICAL SPECIFICATIONS
| Equipment Required |
Microcentrifuge, thermocycler with a heated lid |
| Input |
10 – 500 ng of genomic DNA |
| Sample Sources |
Purified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density. |
| Sequencing Platform Compatible |
Libraries are compatible with all Illumina sequencing platforms except HiSeq X Series. |
| Supplemental Info |
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FORMAT
| Cat # |
Name |
Size |
| D5460 |
Zymo-Seq RRBS Library Kit (24 preps) |
24 Preps |
| D5461 |
Zymo-Seq RRBS Library Kit (48 preps) |
48 Preps |
