RT-qPCR Extraction Control Orange
El control de extracción de RNA no solo permite a los usuarios de un ensayo de diagnóstico de qPCR determinar si hay inhibidores en el ensayo de PCR, sino también validar el éxito del paso de extracción, reduciendo la posibilidad de obtener un resultado falso negativo en la muestra de RNA.
Características de Producto
- Simple: protocolo simplificado para la validación directa de la extracción de ARN y la determinación de la inhibición del ensayo RT-qPCR
- Sensible: el ensayo de control identifica incluso efectos pequeños sobre la extracción de ARN y la inhibición de la amplificación
- Optimizado: el ARN de control tiene una secuencia sin homología conocida con ningún organismo, evitando así la detección de la muestra de ARN
- Específico: ensayo basado en sonda diseñado específicamente para la secuencia de control REC
- Flexible: ideal para usar con una amplia gama de tipos de muestras, incluidas muestras ricas en inhibidores como muestras de sangre, orina y esputo
Descripción de producto
A common practice in qPCR is to add a known amount of spiked control RNA after RNA extraction, this monitors PCR inhibition but has no value as an extraction control. The ideal situation is to have the test sample and internal control undergo the same processing prior to qPCR. Bioline has developed a RT-qPCR Extraction Control, which more closely mimics the test sample, as compared to spike controls. Genetic material from the test sample and the RT-qPCR Extraction Control is simultaneously extracted by common extraction methods, with the extraction control being as sensitive to inhibition and extraction failure as the test sample.
Artificial RT-qPCR Extraction Control cells are of a known concentration, containing the Internal Control RNA sequence. This sequence contains no known homology to any organism and, importantly, has minimal interference with detection of sample RNA. The RT-qPCR Extraction Control cells are spiked into the lysis buffer with the target sample, prior to RNA extraction. Control Mix, which primers and probe, is then added to the reaction mix before amplification. Signal derived from the Internal Control RNA confirms the success of the extraction step. RT-qPCR Extraction Control also monitors co-purification of PCR inhibitors that may cause biased or false amplification patterns.
Código de Producto
| Cat. No. |
Size |
| MDX029 |
500 Reactions |
Aplicaciones
- Pathogen detection
- Cancer risk assessment
- Gene expression analysis
- Drug therapy efficacy
- Biomarker validation
- Copy number variation (CNV) analysis
- Genotyping
- Viral loading
