Sequencing Platform |
NovaSeq 6000™ or NovaSeq X™ |
Read Length |
2 x 150 bp, Unique Dual Indexing (UDI) |
Sequencing Depth |
~30 mln read pairs (can be customized) |
Accepted Sample Types |
RNA, Cells, Tissue, FFPE Tissue Samples |
Library Prep |
Zymo-Seq RiboFree Total RNA Library Kit (cat. R3000) |
rRNA Depletion |
Enzymatic rRNA depletion compatible with any species |
Data Visualization |
Sample similarity matrix, MDS plot, heatmap with top gene expression patterns, scatter plot, MA plot |
Statistical Analysis |
Differential gene expression analysis with DESeq2 package, gene set entrichment analysis (if pathway annotation is available) |