| Sequencing Platform |
NovaSeq 6000™ or NovaSeq X™ |
| Read Length |
2 x 150 bp, Unique Dual Indexing (UDI) |
| Sequencing Depth |
~30 mln read pairs (can be customized) |
| Accepted Sample Types |
RNA, Cells, Tissue, FFPE Tissue Samples |
| Library Prep |
Zymo-Seq RiboFree Total RNA Library Kit (cat. R3000) |
| rRNA Depletion |
Enzymatic rRNA depletion compatible with any species |
| Data Visualization |
Sample similarity matrix, MDS plot, heatmap with top gene expression patterns, scatter plot, MA plot |
| Statistical Analysis |
Differential gene expression analysis with DESeq2 package, gene set entrichment analysis (if pathway annotation is available) |