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Quick-16S Plus NGS Library Prep Kit (V4)

Productos - NGS, Librerias y Servicios - Librerías - Microbiome Sequencing

Quick-16S Plus NGS Library Prep Kit (V4)

Ref. D6430-PS1

HIGHLIGHTS
  • Fast: 8x less hands-on time than conventional 16S library prep protocols.
  • Easy: Premixed Plate minimizes hands-on time and handling errors.
  • Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume!

 

DESCRIPTION

 

The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing.

 

The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process.

 

Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary.

 

With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

 

 

TECHNICAL SPECIFICATIONS

 

Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~388 bp.
Equipment Needed (user provided) Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates.
Index Sequences 10 bp indexes are listed in the MiSeq Sample Sheet Template provided.
Sample Input Purified microbial DNA (≤100 ng), free of PCR inhibitors.
Sequencing Platform Compatible with all Illumina® sequencing platforms using custom sequencing primers. We recommend the MiSeq® Reagent Kit v2 (300-cycle).
V4 Primer Sequences (adapters not included) 515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT).

 

 

FORMAT

 

 

Cat # Name Size
D6430-PS1 Quick-16S Plus NGS Library Prep Kit (V4) with Primer Set 1 96 rxns
D6430-PS2 Quick-16S Plus NGS Library Prep Kit (V4) with Primer Set 2 96 rxns
D6430-PS3 Quick-16S Plus NGS Library Prep Kit (V4) with Primer Set 3 96 rxns
D6430-PS4 Quick-16S Plus NGS Library Prep Kit (V4) with Primer Set 4 96 rxns
D6432 Quick-16S Plus NGS Library Prep Kit (V4) 384 rxns

 



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