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Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI)

Productos - NGS, Librerias y Servicios - Librerías - Microbiome Sequencing

Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI)

Ref. D6421-PS1

HIGHLIGHTS
  • The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
  • 100% automation ready with only a single PCR step and without the need for normalization.
  • Real-time PCR enables absolute microbial copy number quantification.

 

DESCRIPTION

 

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing.

 

The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups.

 

Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary.

 

With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

 

Click here to learn more.

 

TECHNICAL SPECIFICATIONS

 

16S V3-V4 Primer Sequences (adapters not included) 341f (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp) and 806r (GACTACNVGGGTMTCTAATCC, 24 bp). The forward primer 341f is a mixture of the two sequences listed.
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~606 bp.
Barcode Sequences Barcode Sequences: 10 bp barcodes, Available for download here.
Index Primers Unique dual index (barcodes) to label individual samples.
Primer Coverage bacteria (84.2%), archaea (75.8%), and eukaryota (0.0%).
Required Equipment Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample Input Purified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing Platform Illumina MiSeq® without the need to add custom sequencing primers. Zymo Research recommends the MiSeq® Reagent Kit v3 (600-cycle). For assistance with sample sheet setup, see Appendix F.

 

 

FORMAT

 

Cat # Name Size
D6421-PS1 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 1 96 rxns
D6421-PS2 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 2 96 rxns
D6421-PS3 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 3 96 rxns
D6421-PS4 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 4 96 rxns
D6421-PS5 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 5 96 rxns
D6421-PS6 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 6 96 rxns
D6421-PS7 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 7 96 rxns
D6421-PS8 Quick-16S Plus NGS Library Prep Kit (V3-V4 UDI) with Primer Set 8 96 rxns

 



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https://zymoresearch.eu/products/quick-16s-plus-ngs-library-prep-kit-v3-v4-udiLink


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