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Quick-16S Plus NGS Library Prep Kit (V1-V2)

Productos - NGS, Librerias y Servicios - Librerías - Microbiome Sequencing

Quick-16S Plus NGS Library Prep Kit (V1-V2)

Ref. D6434-PS1

HIGHLIGHTS
  • The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
  • 100% automation ready with only a single PCR step and without the need for normalization.
  • Real-time PCR enables absolute microbial copy number quantification.
DESCRIPTION

 

The Quick-16S Plus NGS Library Prep Kit (V1-V2) is the fastest and simplest NGS library prep targeting the V1-V2 region of the 16S rRNA gene for high-throughput sequencing.

 

The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers.

 

After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups.

 

Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

 

Learn More

 

TECHNICAL SPECIFICATIONS

 

16s V1-V2 Primer Sequences (adapters not included) 27f (AGRGTTYGATYMTGGCTCAG, 20 bp) and 341r (CTGCWGCCHCCCGTAGG, 17 bp).
Amplicon Size The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~492 bp.
Barcode Sequences 10 bp barcodes are available to download here or by visiting the Documentation section of this Product Page.
Index Primers Unique dual index (barcodes) to label individual samples.
Required Equipment Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample Input Purified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing Platform Illumina MiSeq® or NextSeq 1000/2000® without the need to add custom sequencing primers. We recommend the MiSeq® Reagent Kit v3 (600-cycle) or NextSeq 1000/2000® Reagent Kit P1/P2 (600-cycle). For assistance with sample sheet setup, see Appendix F.

 



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https://zymoresearch.eu/products/quick-16s-plus-ngs-library-prep-kit-v1-v2Link


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