Productos - NGS, Librerias y Servicios - Librerías - Microbiome Sequencing

Quick-16S Full-Length Library Prep Kit

Ref. D6450-PS1

Quick-16S Full-Length Library Prep Kit

Highlights

Speed – DNA to pooled libraries in 30 minutes of hands-on time.
Simplicity – One-step PCR with premixed amplification plate. Just add DNA.
Flexibility – Compatible with both PacBio and Oxford Nanopore Technologies.

Product Description

The Quick-16S Full-Length Library Prep Kit delivers pooled and purified libraries ready for downstream sequencing on PacBio & Oxford Nanopore sequencing platforms in as little as 30 minutes of hands-on time. Available in four uniquely barcoded formats, 96-well premixed reagent plates allow users to simply add DNA and amplify while providing the capacity to multiplex up to 384 samples. The PCR premix in each plate is qPCR compatible, contains sufficient dead volume for automated liquid handling, and uses highly stable reagents that allow for room temperature PCR setup without the use of ice or cold-blocks.

Technical Specifications

Sample Input	Normalized and purified microbial DNA, free of PCR inhibitors.
Full-Length 16S Primer Sequences	27f (AGRGTTYGATYMTGGCTCAG, 20 bp) and 1492r (RGYTACCTTGTTACGACTT, 19 bp). Sequencing adapters are included but not shown.
Primer Indexes	Combinational dual indexes (barcodes) to uniquely label samples.
Barcode Sequences	10 bp barcodes are available for download by visiting the documentation section of the D6450 Product Page at www.zymoresearch.com.
Amplicon Size	The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~1500 bp.
Sequencing Platform	PacBio Sequel^® II, Sequel^® IIe, and Revio^™ systems. ONT PromethION^®, GridION^®, and MinION^®.
Equipment Needed (user provided)	Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible, recommended), or standard PCR system, 96-well real-time PCR plates, and PCR plate adhesive seals. Use of a magnetic tube rack or plate rack is highly recommended.

ORDER

Cat #	Name	Size
D6450-PS1	Quick-16S Full-Length Library Prep Kit with Primer Set 1	96 rxns
D6450-PS2	Quick-16S Full-Length Library Prep Kit with Primer Set 2	96 rxns
D6450-PS3	Quick-16S Full-Length Library Prep Kit with Primer Set 3	96 rxns
D6450-PS4	Quick-16S Full-Length Library Prep Kit with Primer Set 4	96 rxns