Características de Producto
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ADN humano purificado, no metilado y metilado para su uso como controles negativos y positivos en aplicaciones de detección de metilación.
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Los estándares se pueden analizar en paralelo con muestras para monitorear la eficiencia de conversión de bisulfito.
Controles ideales para PCR de secuenciación de bisulfito (BSP) y PCR específica de metilación (MSP).
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Cada estándar se proporciona con un juego de cebadores para amplificar un fragmento de ADN después de la conversión de bisulfito.
Descripción de Producto
The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation family of products. This DNA set can be included as a positive and negative control to assess the efficiency of bisulfite conversion of DNA. The non-methylated human DNA is purified from the HCT116 DKO cell line, which contains genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-). The methylated DNA standard is purified HCT116 DKO DNA that has been enzymatically methylated at CpG sites.
Especificaciones Técnicas
| Concentration |
250 ng/µl |
| Format |
Purified genomic DNA |
| Storage |
≤ -20°C |
Código de Producto
| Cat # |
Name |
Size |
| D5014-1 |
Human HCT116 DKO Non-Methylated DNA |
5 µg/20 µl |
| D5014-2 |
Human HCT116 DKO Methylated DNA |
5 µg/20 µl |
| D5014 |
Human Methylated & Non-methylated DNA Set |
5 µg/20 µl |
