DESCRIPTION
The Primerdesign genesig Advanced Kit for Monkeypox is designed for the quantitative and qualitative detection of the monkeypox genome from DNA extracted from human serum, lesion exudate, or scab samples from human/animal patients of all ages.
The patient may be exhibiting signs and symptoms of monkeypox virus infection at any point during infection or be being screened after identification as a potential close contact.
The Primerdesign genesig Advanced Kit for Monkeypox is configured for use with any real-time PCR instrument equipped to detect amplification in the FAM and VIC/HEX channels. The kit is designed to have a broad detection profile.
Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences of all Monkeypox viral clades available at the time of design (May 2022).
STORAGE
This kit is stable at room temperature but should be stored at -20ºC on arrival. Once the lyophilised components have been resuspended, they should not be exposed to temperatures above -20°C for longer than 30 minutes at a time and unnecessary repeated freeze/thawing should be avoided.
The kit is stable for six months from the date of resuspension under these circumstances. If a standard curve dilution series is prepared for quantitative detection, this can be stored frozen for an extended period. If you see any degradation in this serial dilution a fresh standard curve can be prepared from the positive control. Primerdesign does not recommend using the kit after the expiry date stated on the pack.
SUITABLE SAMPLE MATERIAL
All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and DNA integrity. Always run at least one negative control with the samples. To prepare a negative control, replace the template DNA sample with RNase/DNase-free water.
DYNAMIC RANGE OF TEST
Under optimal PCR conditions, genesig detection kits have very high priming efficiencies of >90% and can detect less than 100 copies of the target template
