PRODUCT FEATURES
- Exceptional value for money
- Rapid detection of all clinically relevant subtypes
- Positive copy number standard curve for quantification
- Highly specific detection profile
- High priming efficiency
- Broad dynamic detection range (>6 logs)
- Sensitive to < 100 copies of target
- Accurate controls to confirm findings
SPECIFICITY
The Primerdesign genesig Kit for Escherichia coli O157:H7 (E.coli_O157) genomes is designed for the in vitro quantification of E.coli_O157 genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design.
The dynamics of genetic variation means that new sequence information may become available after the initial design. Primerdesign periodically reviews the detection profiles of our kits and when required releases new versions.
This primers used to develop this kit have been adapted from the recent paper below: Li et al. BMC Microbiology (2017) 17:215; Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli.
