Productos - Biología molecular - Proteínas - Enzimas

DNase I Set

Ref. E1010

DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA.

Características de Producto

Lyophilized enzyme provided with DNA Digestion Buffer

Descripción de Producto

DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The DNase I Set features lyophilized enzyme and DNA Digestion Buffer.

Especificaciones Técnicas

Concentration	1 U/µl
Heat inactivation	Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min..
Included	One unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl₂, and 10 mM CaCl₂). One unit of enzyme is equivalent to one Kunitz unit.
Resuspension Volume	275 µl
Size	250 U