16S/ITS Amplicon Sequencing Service

Identification of Bacteria, Archaea, & Fungi From Any Microbiome Sample

Comprehensive Bioinformatics Analysis

Simplify 16S/ITS Amplicon Sequencing Data Interpretation

All projects include a comprehensive report with bioinformatics analysis and raw sequencing data. The 16S rRNA sequencing service report provides compositional bar plots, taxonomy heatmaps, alpha-diversity and beta diversity analyses. Group comparisons and LEfSe biomarker discovery are also reflected in all 16S/ITS amplicon sequencing service reports. Our team of expert bioinformaticians are here to help you get the most out of your data.

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Achieve Species-Level Identification With 16S Amplicon Sequencing

By combining leading edge sequencing error correction methods with a highly curated 16S rRNA database, our 16S amplicon sequencing service can provide taxonomic identification down to the species level.

Gain Unique Insights Into Your Data

Zymo Research's Absolute Abundance Quantification Reveals More

Relative abundance alone cannot define changes in a microbial community. In cases like comparing treatment or therapeutic effects, it can be just as important to know how cell numbers are changing. With absolute abundance quantification provided by our 16S amplicon sequencing service, open new insights into the microbial community dynamics of your project.

Rigorously Optimized For The Highest Data Accuracy

Our 16S amplicon sequencing service is continually monitored with mock community controls to ensure data accuracy and consistency. Don’t let run-to-run variations interfere with your long-term study or project.

16S/ITS Amplicon Sequencing Made Simple

How Our Straightforward, User-Friendly Service Works

16S/ITS Amplicon Sequencing Technical Specifications

Frequently Asked Questions

Do you also offer ITS and 18S sequencing services? Yes. The sample requirements and submission guidelines are the same as for our 16S Sequencing Service.

Which sample types are accepted for the 16S Sequencing Service? We have processed a wide variety of sample types, from human tissue samples, environmental samples, to food samples. If you have questions or concerns, please feel free to fill out an inquiry or contact us.

What are the requirements for raw samples? We recommend sending 200-500 mg for solid samples or 500-1mL of liquid samples in general. This may vary depending on the specific sample type.

What are the requirements for DNA samples? The minimum volume of DNA required is 30 µl with a recommended concentration of 10-50 ng/µl. If the concentration is lower, we can often still successfully sequence as our pipeline is quite robust.

Is there a minimum sample number requirement? There is no sample minimum required in order to submit to our 16S Sequencing Service.

How do you control for run-to-run sequencing variation? Because of our included quality control standard, it is possible to batch samples over time and have confidence that the samples were processed in the same way. We recently completed a project that had a total of >2,000 samples processed over a period of 6 months. By comparing the results from the standard for each group of samples, we had confidence that the results from different batches could be compared together.

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