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GENÓMICA

CÉLULAS COMPETENTES

ELECTROFORESIS

ELISAS

EPIGENÓMICA

EXOSOMAS HANSABIOMED

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVO QUIMICO/BIOQUIMICO

TRANSFECCIÓN

TRANSLOCACIONES LEUCÉMICAS HEMAVISION

PROTEÓMICA

NGS / MICROARRAYS

INSTRUMENTACIÓN

CONSUMIBLES


 
Xpedition™ Plant/Seed MiniPrep
 
  • "Take the Lab to the Field" and to the site of sample collection with state of the art Xpedition™ Sample Prep and DNA preservation technologies.
  • For efficient BashingBead™ lysis and isolation of inhibitor-free, PCR-quality DNA directly from tough-to-lyse plant and seed samples.
  • Unique lysis/stabilization solution keeps DNA intact in lysates for storage and transport following remote sample processing with the Xpedition™ Sample Processor (XSP).

Degradation and contamination of biological samples have been obstacles to scientific study, and may be particularly problematic in highly sensitive molecular-analysis techniques (e.g., PCR of low copy DNA). Use of cryogenic freezing methods for environmental/forensic sample preservation may often be too impractical to be employed.

The Xpedition™ Plant/Seed DNA MiniPrep, when used with a portable bead beating device [i.e., Xpedition™ Sample Processor (XSP), pg. 6], allows the researcher/investigator to "Take the Lab to the Field" and to the site of sample collection.

The Xpedition™ Plant/Seed DNA MiniPrep is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. Samples from remote locations can be processed at the site of collection using the provided ZR BashingBead™ Lysis Tubes and a XSP (available separately, Cat. No. S6020, pg. 6). Sample processing occurs in a unique lysis/stabilization solution that effectively preserves DNA in plant lysates for subsequent storage and transport. Later, when convenient with the researcher, the kit´s Fast-Spin column technologies are used for the isolation of polyphenol, lipid, polysaccharide-free DNA from the lysates.

Eluted DNA is ideal for PCR for genotypic analysis including metagenomics, barcoding, phylogenetics, forensics, etc. An overview of the procedure is shown below.

Format Bead Beating, Spin Column
DNA Recovery Typically, up to 25 μg total DNA is eluted into 50-100 μl (25 μl minimum) DNA Elution Buffer per sample
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer (optional)
DNA Size Limits Capable of recovering genomic DNA up to and above 40 Kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered
Sample Source Up to 150 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.
DNA Purity High quality, inhibtor-free DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. A260/280 >1.8
DNA Yield Typically 20-80 ng DNA/mg plant material

 

Product Name Size Ref.:

Xpedition™ Plant/Seed DNA MiniPrep

50 Preps. D6221
 

     Información adicional del producto
     Xpedition™ Plant/Seed DNA MiniPrep
     Cantidad:

     Archivos Adjuntos :
Ficha Seguridad Xpedition Plant
Protocolo Xpedition Plant



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E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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