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GENÓMICA

CÉLULAS COMPETENTES

ELECTROFORESIS

ELISAS

EPIGENÓMICA

EXOSOMAS HANSABIOMED

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVO QUIMICO/BIOQUIMICO

TRANSFECCIÓN

TRANSLOCACIONES LEUCÉMICAS HEMAVISION

PROTEÓMICA

NGS / MICROARRAYS

INSTRUMENTACIÓN

CONSUMIBLES


 
ZR-96 Plant/Seed DNA Kit™
 
  • Simple method for the isolation of DNA from thoug-to-lyse plant and seed samples in minutes
  • Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
  • Fast-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

The ZR-96 plant/seed DNA Kits are designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Fast-Spin column technology. The eluted DNA is filteree to remove polyphenolics making it ideal for downstream molecular-based applicatinos including PCR, arrays, etc.

Format 96 well plate
DNA Recovery

Up to 5 μg total DNA is eluted in ≥25 μl DNA Elution Buffer per sample.

Processing Time

50 minutes

Equipment

Centrifuge w/microplate carriers, 96-well Disruptor/Pulverizer

DNA Size Limits

Capable of recovering genomic DNA size fragments from 100 bp to ≥40 Kb. Typical fragments sizes range from 25 to 35 Kb. If present, parasitic and irla DNA will also be recovered.

Sample Sources

Up to 80 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.

DNA Purity

High quality, inhibitor-fee DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. A260/280 > 1.8

DNA Yield

Typically 20-80 ng DNA/ng Plant material is recovered

 

Comparison of DNA yields from varios plant and seed samples using the ZR Plant/Seed DNA kits. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0,8% (w/v) agarose/ethidium bromide gel. M isa a 1 Kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), Juniper (2), Corn Kernel (3, 4), Sunflower seed (5, 6).

 

Product name Size Ref.:

ZR-96 Plant/Seed DNA Kit™

2x96 D6021
 

     Información adicional del producto
     ZR-96 Plant/Seed DNA Kit™
     Cantidad:

     Archivos Adjuntos :
Ficha Seguridad ZR 96 Plant
Protocolo ZR 96 Plant



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Nuestros distribuidores

Ptge. Dos de Maig, 9-11 . 08041 Barcelona
E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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