HIHGHLIGHTS

• Methylated DNA enrichment for large-scale DNA methylation analysis.
• A highly specific anti-5-methylcytosine monoclonal antibody for defined, reproducible results.
• Includes control DNA and primers for easy monitoring of the entire procedure.
• Eluted, ultra-pure DNA is ideal for use in subsequent molecular based analyses (e.g., assembling genomic libraries and determining genome-wide methylation status).

SPECIFICATIONS

• Application: The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5 methylcytosine-containing DNA with a highly specific anti-5-methylcytosine monoclonal antibody for the capture and separation of methylated DNA from non- methylated DNA in only a few hours, from any pool of fragmented genomic DNA for use in genome-wide methylation analysis.
• Components: The Methylated-DNA IP Kit comes as a two component kit: the kit box along with a separate icebox containing the cold-storage components.
• Enrichment Factor: > 100 fold for Methylated versus Non-methylated DNA.
• Elution: Ultra pure, enriched methylated DNA can be eluted with 10 µl Elution Buffer or water.

DESCRIPTION

The Methylated-DNA IP Kit utilizes IP technology for the enrichment of 5-methylcytosine-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. The kit features a highly specific anti-5-methylcytosine monoclonal antibody for the capture and separation of methylated DNA from non-methylated DNA in only a few hours (see figure below). Typically, over a hundred-fold enrichment of methylated DNA vs. non-methylated DNA can be achieved with the use of this kit. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including: PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing and microarray.