The Universal Methylated DNA Standard includes enzymatically methylated DNA together with a specially-designed primer set to be used in conjunction with Zymo Researchs EZ DNA Methylation™, EZ DNA Methylation-Gold™, and EZ DNA Methylation-Direct™ Kits to assess the efficiency of bisulfite-mediated conversion of DNA. Central to this, is the pUC19 DNA that was isolated from a methylation-negative strain of bacteria (dam-, dcm-) prior to its enzymatic modification with Sss I methylase (EC 2.1.1.73). The DNA is methylated at cytosine positions comprising CG dinucleotides. The primer set herein has been designed to amplify a fragment of the supplied pUC19 DNA following bisulfite treatment. The methylated cytosines comprising CG dinucleotides remain unconverted following bisulfite treatment, whereas nonmethylated cytosines are converted into uracil and detected as thymine following PCR.
Universal Methylated DNA Standard, 20 µl. This standard contains pUC19 DNA, which contains methylated cytosines (C5) at all CG dinucleotide positions.
