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AMPLIFICACIÓN (PCR) > AMPLIFICACIÓN RNA > TargetAmp™ aRNA Amplification Kits

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AMPLIFICACIÓN RNA

TargetAmp™ aRNA Amplification Kits

The TargetAmp Kits set new standards in small sample RNA amplification...
because the best microarray data comes from small samples.

TargetAmp Citations

Purify total RNA from 1 - 10,000 cells.

Sensitive qRT-PCR from a single cell.

Biotin-X-X-NHS for labeling aminoallyl-aRNA or aminoallyl-cDNA.

The TargetAmp aRNA Amplification Kits set new standards in small sample RNA amplification for microarray studies. The kits, based on the linear RNA amplification process developed in the laboratory of Dr. James Eberwine, offer:

High yields of aRNA (cRNA) from small samples.
- Produce microgram amounts of aRNA (cRNA) from 10 pg (1 cell) of total RNA using the TargetAmp 2-Round aRNA Amplification Kits.
- Produce microgram amounts of aRNA (cRNA) from 25 ng of total RNA using the TargetAmp 1-Round aRNA Amplification Kits.
Fast and easy reactions.
Reproducible RNA amplification and microarray results.
High correlation with Affymetrix standard labeling procedure.
High quality results using Affymetrix GeneChip®, Illumina BeadChip and other commercial and self-spotted microarray platforms.

Choose the TargetAmp Kit for your needs

For more details, check the TargetAmp protocols	1-Round Amplification			2-Round Amplification
For more details, check the TargetAmp protocols	TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101	TargetAmp™ 1-Round aRNA Amplification Kit 103	TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104	TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0	TargetAmp™ 2-Round aRNA Amplification Kit 2.0
Starting Total RNA per Reaction	25 ng – 500 ng	25 ng – 500 ng	25 ng – 500 ng	10 pg – 500 pg	10 pg – 500 pg
Typical Yield of Amplified aRNA	3 µg – 60 µg	3 µg – 60 µg	3 µg – 60 µg	1.5 µg – 70 µg	1.5 µg – 70 µg
Reverse Transcriptase(s) Used	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)	SuperScript™ III & SuperScript™ II (provided by the user)
Fold Amplification	>5000	>5000	>5000	>5,000,000	>5,000,000
Time Required	6 hours	6 hours	6 hours	2 days	2 days
End Product	Aminoallyl-aRNA	Unlabeled-aRNA	Biotin-aRNA	Aminoallyl-aRNA	Unlabeled-aRNA
Target Label	Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)	Biotin-aRNA (Biotin-UTP is included in this kit)	Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user)	Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)

Product Catalog No. Size
TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101 TAA1R4910
10 Rxns
bbb TAA1R4924
24 Rxns
Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
Greater than 5000-fold amplification.
Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
bbb bbb
TargetAmp™ 1-Round aRNA Amplification Kit 103 TAU1R5110
10 Rxns
bbb TAU1R5124
24 Rxns
Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
Greater than 5000-fold amplification.
Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
bbb bbb
TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104 TAB1R6910
10 Rxns
bbb TAB1R6924
24 Rxns
Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
Greater than 5000-fold amplification.
Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
bbb bbb
TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 TAA2R4910
10 Rxns
bbb TAA2R4924
24 Rxns
Produces microgram amounts of Aminoallyl-aRNA from 10 pg – 500 pg total RNA.
Greater than 5,000,000-fold amplification.
Optimized for use with SuperScript™ III &
SuperScript™ II Reverse Transcriptase provided by the user).
bbb bbb
TargetAmp™ 2-Round aRNA Amplification Kit 2.0
TAU2R5110
10 Rxns
bbb
TAU2R51224
24 Rxns
Produces microgram amounts of unlabeled-aRNA from 10 pg – 500 pg total RNA.
Greater than 5,000,000-fold amplification.
Optimized for use with SuperScript™ III &
SuperScript™ II Reverse Transcriptase (provided by the user).
bbb bbb

Información adicional del producto


TargetAmp 1-Round Kit 101


TargetAmp 1-Round Kit 103


TargetAmp 1-Round Kit 104


TargetAmp 2-Round Kit 1.0


TargetAmp 2-Round Kit 2.0

Cantidad:

Archivos Adjuntos :

	Protocolo kit 101
	Protocolo kit 103
	Protocolo kit 104
	Protocolo 2-round kit 1.0
	Protocolo 2-round kit 2.0

nº de productos: 0

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