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GENÓMICA

ANÁLISIS METILACIÓN

ELECTROFORESIS

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVOS QUÍMICOS

PROTEÓMICA

DIAGNÓSTICO

INSTRUMENTACIÓN

CONSUMIBLES


 
TargetAmp™ aRNA Amplification Kits
 

Evaluation

TargetAmp Citations

Purify total RNA from 1 - 10,000 cells.

Sensitive qRT-PCR from a single cell.

Biotin-X-X-NHS for labeling aminoallyl-aRNA or aminoallyl-cDNA.

The TargetAmp aRNA Amplification Kits set new standards in small sample RNA amplification for microarray studies. The kits, based on the linear RNA amplification process developed in the laboratory of Dr. James Eberwine, offer:

  • High yields of aRNA (cRNA) from small samples.
    • Produce microgram amounts of aRNA (cRNA) from 10 pg (1 cell) of total RNA using the TargetAmp 2-Round aRNA Amplification Kits.
    • Produce microgram amounts of aRNA (cRNA) from 25 ng of total RNA using the TargetAmp 1-Round aRNA Amplification Kits.
  • Fast and easy reactions.
  • Reproducible RNA amplification and microarray results.
  • High correlation with Affymetrix standard labeling procedure.
  • High quality results using Affymetrix GeneChip®, Illumina BeadChip and other commercial and self-spotted microarray platforms.

Choose the TargetAmp Kit for your needs

For more details, check the TargetAmp protocols 1-Round Amplification 2-Round Amplification
TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101

TargetAmp™ 1-Round aRNA Amplification Kit 103

TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104 TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0
TargetAmp™ 2-Round aRNA Amplification Kit 2.0
Starting Total RNA per Reaction 25 ng – 500 ng 25 ng – 500 ng 25 ng – 500 ng 10 pg – 500 pg 10 pg – 500 pg
Typical Yield of Amplified aRNA 3 µg – 60 µg 3 µg – 60 µg 3 µg – 60 µg 1.5 µg – 70 µg 1.5 µg – 70 µg
Reverse Transcriptase(s) Used SuperScript™ III (provided by the user) SuperScript™ III (provided by the user) SuperScript™ III (provided by the user) SuperScript™ III & SuperScript™ II (provided by the user) SuperScript™ III & SuperScript™ II (provided by the user)
Fold Amplification >5000 >5000 >5000 >5,000,000 >5,000,000
Time Required 6 hours 6 hours 6 hours 2 days 2 days
End Product Aminoallyl-aRNA Unlabeled-aRNA Biotin-aRNA Aminoallyl-aRNA Unlabeled-aRNA
Target Label Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user) Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user) Biotin-aRNA (Biotin-UTP is included in this kit) Biotin-NHS, Cy™-NHS, or other-NHS (provided by the user) Biotin-NTP, Cy™-NTP, or other-NTP (provided by the user)
Product Catalog No. Size
TargetAmp™ 1-Round Aminoallyl-aRNA Amplification Kit 101 TAA1R4910

10 Rxns

bbb TAA1R4924

24 Rxns

  • Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
  • Greater than 5000-fold amplification.
  • Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
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TargetAmp™ 1-Round aRNA Amplification Kit 103 TAU1R5110

10 Rxns

bbb TAU1R5124

24 Rxns

  • Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
  • Greater than 5000-fold amplification.
  • Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
bbb bbb
TargetAmp™ 1-Round Biotin-aRNA Amplification Kit 104 TAB1R6910

10 Rxns

bbb TAB1R6924

24 Rxns

  • Produces microgram amounts of aminoallyl-aRNA from 25 ng – 500 ng total RNA.
  • Greater than 5000-fold amplification.
  • Optimized for use with SuperScript™ III Reverse Transcriptase (provided by the user).
bbb bbb
TargetAmp™ 2-Round Aminoallyl-aRNA Amplification Kit 1.0 TAA2R4910

10 Rxns

bbb TAA2R4924

24 Rxns

  • Produces microgram amounts of Aminoallyl-aRNA from 10 pg – 500 pg total RNA.
  • Greater than 5,000,000-fold amplification.
  • Optimized for use with SuperScript™ III &
    SuperScript™ II Reverse Transcriptase provided by the user).
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TargetAmp™ 2-Round aRNA Amplification Kit 2.0

 TAU2R5110

10 Rxns

bbb

TAU2R51224

24 Rxns

  • Produces microgram amounts of unlabeled-aRNA from 10 pg – 500 pg total RNA.
  • Greater than 5,000,000-fold amplification.
  • Optimized for use with SuperScript™ III &
    SuperScript™ II Reverse Transcriptase (provided by the user).
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     Información adicional del producto
     TargetAmp 1-Round Kit 101
     TargetAmp 1-Round Kit 103
     TargetAmp 1-Round Kit 104
     TargetAmp 2-Round Kit 1.0
     TargetAmp 2-Round Kit 2.0
     Cantidad:

     Archivos Adjuntos :
Protocolo kit 101
Protocolo kit 103
Protocolo kit 104
Protocolo 2-round kit 1.0
Protocolo 2-round kit 2.0


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E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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