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GENÓMICA

ANÁLISIS METILACIÓN

ELECTROFORESIS

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVOS QUÍMICOS

PROTEÓMICA

DIAGNÓSTICO

INSTRUMENTACIÓN

CONSUMIBLES


 
Mouse Normalisation Gene Panel
 

Despite the popularity of quantitative PCR, there are many factors that the researcher must consider when planning experiments and one of these is to normalise for variations in the amount of template material. An ideal control is one that is unaffected by all conditions or treatments. Several strategies for normalisation have been proposed including the use of total input RNA, cell number and rRNA. The most popular method is normalisation to an appropriate internal reference gene.

Quantace has developed a Mouse Normalisation Gene Panel using SYBR® Green I chemistry. The panel comprises primer sets for 12 candidate reference genes from functionally diverse families (see Table below).

Primer
pair
Cat.no.GeneEnsemble id / genbankProduct
spans
exons
 SSDHAQT12X
MS-01
Succinate
dehydrogenase
complex,
subunit A

ENSMUSG00000021577

14 and 15

GACTINQT12X
MS-02
Gamma actin,
cytoplasmic

ENSMUSG00000062825

5 and 6

YWHAZQT12X
MS-03
tyrosine 3-monooxygenase, zeta polypeptide

ENSMUSG00000022285

Not intron
spanning

RPS18QT12X
MS-04
Ribosomal
Protein S18

ENSMUSG00000008668

1 and 2

 GAPDHQT12X
MS-05
Glyceraldehyde-
3-phosphate

ENSMUSG00000057666

4 and 5

 B2MGQT12X
MS-06
Beta-2
microglobulin

 ENSMUSG00000060802

 2 and 3

 RPL13AQT12X
MS-07
Ribosomal
protein L13a

 ENST00000270634

 10 and 11

 PGKQT12X
MS-08
Phosphoglycerate
kinase 1

 ENSMUSG00000062070

 9 and 10

 CALNXQT12X
MS-09
Calnexin

 ENSMUSG00000020368

 1 and 2

 BACTINQT12X
MS-10
Beta actin
cytoplasmic

 ENSMUSG00000029580

 3 and 4

 RN18SQT12X
MS-11
18S rRNA

 Genbank (X56974)

 intronless
gene

 UBQCQT12X
MS-12
Ubiquitin C

 ENSMUSG00000008348

 1 and 2

Identification of the most appropriate reference gene is achieved by comparing the gene of interest against the Mouse Normalisation Panel, using standard analysis packages such as GeNorm (Vandesompele et al. 2002), BestKeeper (Pfaffl et al. 2004) or Normfinder (Andersen et al. 2004). These primer sets have been optimised for use with the SensiMix range of reagents. The use of SensiMix with this gene panel results in amplification efficiencies of between 95 and 102% along with excellent R, R2 and M values and an absence of primer dimers.

These primers can be ordered as a set of 12 or as individual primer sets. An application note containing ideal results is supplied with the full panel but can be downloaded as a pdf file here.

House-Keeping GenesPack SizeRef.:
Mouse Normalisation
Gene Panel
12 x 100 rxns

QT12X-MS

Mouse Gene, SSDHA

100 rxns

QT12X-MS-01

Mouse Gene, GACTIN

100 rxns

QT12X-MS-02

Mouse Gene, YWHAZ

100 rxns

QT12X-MS-03

Mouse Gene, RPS18

100 rxns

QT12X-MS-04

Mouse Gene, GAPDH

100 rxns

QT12X-MS-05

Mouse Gene, B2MG

100 rxns

QT12X-MS-06

Mouse Gene, RPL13A

100 rxns

QT12X-MS-07

Mouse Gene, PGK

100 rxns

QT12X-MS-08

Mouse Gene, CALNX

100 rxns

QT12X-MS-09

Mouse Gene, BACTIN

100 rxns

QT12X-MS-10

Mouse Gene, RN18S

100 rxns

QT12X-MS-11

Mouse Gene, UBQC

100 rxns

QT12X-MS-12

* 1 reaction = 1µl in 50 µl total volume
 

     Información adicional del producto
     Mouse Gene Panel (Complete)
     Mouse Gene
     Cantidad:



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