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SensiMix (TM) SYBR & Fluorescein Kit/ Real Time
 

SensiMix™ SYBR® & Fluorescein Kit

SensiMix SYBR® & Fluorescein Kit is designed for superior sensitivity and specificity on real-time instruments that measure fluorescein signal as a passive reference for normalization. The mix has been developed to provide the high sensitivity required for demanding assays, or for those assays where template is limited.

Features & Benefits

  • Sensitivity - allows reproducible detection of low-copy number templates
  • Reproducibility - generates real-time data reliably and reproducibly
  • Specific - proprietary hot-start chemistry minimizes non-specific amplification
  • Flexible - for block-based instruments where fluorescein is required to allow calculation of dynamic well factors

Instrument Compatibility

Bio-Rad iCycler®, iQTM5 and MyiQ™. (See product selection table). However the kit can is also compatible with several instruments that do not require the use of fluorescein, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.

Description

SensiMix SYBR® & Fluorescein Kit is a pre-mixed high performance product designed for superior sensitivity and specificity (Fig. 1) on various real-time instruments in which a Fluorescein signal is required. Powered by a proprietary, highly-optimized, chemical hot-start PCR enzyme, the mix has been developed to provide the high sensitivity and specificity required for demanding assays or for those assays where template is limited.

SensiMix SYBR® & Fluorescein is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and enhancers.

For expression analysis, SensiFAST cDNA Synthesis Kit is the perfect partner for SensiMix SYBR® & Fluorescein Kit for very high-quality first strand cDNA synthesis and real-time results.

Components

Reagent

 

500 x 50µl Reactions

2000 x 50µl Reactions

SensiMix™ SYBR® & Fluorescein (2x)

 

10 x 1.25ml    (12.5ml)

40 x 1.25ml         (50ml)

50mM MgCl2

 

1 x 1ml

4 x 1ml

 Volume

  • QT615-05: 500 x 50µl Reactions: 10 x 1.25ml
  • QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml

 

Storage & Stability

All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.

When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

Citations

  1. A signature of immune function genes associated with recurrence-free survival in breast cancer patients
    Ascierto M.L., Kmieciak M., Idowu M.O., Manjili R., Zhao Y., Grimes M., Dumur C., Wang E., Ramakrishnan V., Wang X.Y., Bear H.D., Marincola F.M., Manjili M.H.
  2. Induced Wnt5a expression perturbs embryonic outgrowth and intestinal elongation, but is well-tolerated in adult mice
    Bakker E.R., Raghoebir L., Franken P.F., Helvensteijn W., van Gurp L., Meijlink F., van der Valk M.A., Rottier R.J., Kuipers E.J., van Veelen W., Smits R.
  3. Human Monocytes Undergo Excessive Apoptosis following Temozolomide Activating the ATM/ATR Pathway While Dendritic Cells and Macrophages Are Resistant
    Bauer M., Goldstein M., Heylmann D., Kaina B.
  4. Anergic CD8+ T Lymphocytes Have Impaired NF-?B Activation with Defects in p65 Phosphorylation and Acetylation
    Clavijo P.E., Frauwirth K.A.
  5. Secreted Factors of Human Liver-Derived Mesenchymal Stem Cells Promote Liver Regeneration Early After Partial Hepatectomy
    Fouraschen S.M., Pan Q., de Ruiter P.E., Farid W.R., Kazemier G., Kwekkeboom J., Ijzermans J.N., Metselaar H.J., Tilanus H.W., de Jonge J., van der Laan L.J.

 

ORDERING INFORMATION

Pack Size Presentation Ref.:

500 rxns*

10x1.25 ml

QT615-05

2000 rxns*

40x1.25 ml

QT615-20

* 1 reaction=50 µl final volume

 

     Información adicional del producto
     SensiMixPlus SYBR ¬ Fluorescein
     Cantidad:

     Archivos Adjuntos :
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E-mail: ecogen@ecogen.com



San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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