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GENÓMICA

ANÁLISIS METILACIÓN

ELECTROFORESIS

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVOS QUÍMICOS

PROTEÓMICA

DIAGNÓSTICO

INSTRUMENTACIÓN

CONSUMIBLES


 
SensiMix Two-Step kit
 

Combine the properties of SensiMix and the power of our highly stable Reverse Transcriptase with our one- and two-step kits.

The SensiMix Twe-Step kit is formulated to deliver cDNA synthesis and subsequent amplification in two separate steps. It contains an internal reference and has been designed to work across a range of block and rotor-based instruments.

FEATURES

Increased Thermal Stability

Perform cDNA synthesis at high temperatures (45-55ºC), suited to GC and structure-rich RNA templates.

Reduced Degradation

Addition of RNase Inhibitor to the reaction mix reduces degradation of mRNA or total RNA during cDNA syntehsis

Increased Yields

The use of a two step protocol allows the generation of cDNA and its subsequent use inmany amplification reactions.

Enhanced Sensitivity

Improved efficenciy of reverse transcription and increased amplification make this kit particularly well suited to genes expressed at low levels.

Versatility

  • Universal: can be used for SYBR® Green I or probe-based reactions and has been designed to work across a range of block and rotor-based instruments.
  • SensiMix Two-Step contains an internal reference dye for normalisation.

Improved Specificity

Eliminates non-specific, primer dimers and misprimed products

Small Pack Sizes

Low cost trial-size packs (25 reactions) available

COMPONENTS

2 x Reaction mix consisting of:

  • Heat-activated DNA polymerase
  • Sensi reverse transcriptase
  • 99% pure dNTPs
  • MgCl2 (6mM)

Plus separate vials of:

  • SYBR® Green I
  • 50mM MgCl2
  • RNase inhibitor
  • DEPC-treated water
  • Sensi reverse transcriptase
  • 5x reverse transcription buffer
  • Oligo (dT)12 primer mix
  • Random hexamer primer mix

STORAGE

6 months at -20ºC
Avoid exposure of the SYBR® Green I to light.

ORDERING INFORMATION

Pack SizePresentationRef.:

250 rxns*

5x625 µl

QT305-01

250 rxns*

5x1.25 ml

QT305-02

500 rxns*

10x1.25 ml

QT305-05

2000 rxns*

40x1.25 ml

QT305-20

* 1 reaction=20 µl total volume for RT reaction and 50 µl total volume for fluorescence reaction.

 
     Cantidad:



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Nuestros distribuidores

Ptge. Dos de Maig, 9-11 . 08041 Barcelona
E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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