Combine the properties of SensiMix and the power of our highly stable Reverse Transcriptase with our one- and two-step kits. The SensiMix One-Step kit is formulated to deliver cDNA synthesis and subsequent amplification in a single step. It contains an internal reference and has been designed to work across a range of block and rotor-based instruments. FEATURES
Increased Thermal Stability Perform cDNA synthesis at high temperatures (45-55ºC), suited to GC and structure-rich RNA templates. Reduced Degradation
Addition of RNase Inhibitor to the reaction mix reduces degradation of mRNA or total RNA during cDNA syntehsis Improved Efficiency The One-Step kit generates an accurate linear standard curve from a 1´-fold total ENA dilution series ranging from 1 ug to 10 pg. Versatility - Universal: can be used for SYBR® Green I or probe-based reactions and has been designed to work across a range of block and rotor-based instruments.
- SensiMix One-Step contains an internal reference dye for normalisation.
Improved Specificity Eliminates non-specific, primer dimers and misprimed products Easy-to-use Two reactions performed in one tube! Small Pack Sizes Low cost trial-size packs (25 reactions) available COMPONENTS
2 x Reaction mix consisting of: - Heat-activated DNA polymerase
- Sensi reverse transcriptase
- 99% pure dNTPs
- MgCl2 (6mM)
- Internal reference
Plus separate vials of: - SYBR® Green I
- 50mM MgCl2
- RNase inhibitor
- DEPC-treated water
STORAGE
6 months at -20ºC
Avoid exposure of the SYBR® Green I to light. ORDERING INFORMATION | Pack Size | Presentation | Ref.: | 250 rxns* | 5x625 µl | QT205-01 | 250 rxns* | 5x1.25 ml | QT205-02 | 500 rxns* | 10x1.25 ml | QT205-05 | 2000 rxns* | 40x1.25 ml | QT205-20 |
* 1 reaction=20 µl total volume for RT reaction and 50 µl total volume for fluorescence reaction. |
