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GENÓMICA

CÉLULAS COMPETENTES

ELECTROFORESIS

ELISAS

EPIGENÓMICA

EXOSOMAS HANSABIOMED

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVO QUIMICO/BIOQUIMICO

TRANSFECCIÓN

TRANSLOCACIONES LEUCÉMICAS HEMAVISION

PROTEÓMICA

NGS / MICROARRAYS

INSTRUMENTACIÓN

CONSUMIBLES


 
ZR Plant/Seed DNA Kit™-Extracc. DNA Planta/Semilla
 
  • Simple method for the isolation of DNA from thoug-to-lyse plant and seed samples in minutes
  • Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
  • Fast-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.

The ZR plant/seed DNA Kits are designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Fast-Spin column technology. The eluted DNA is filteree to remove polyphenolics making it ideal for downstream molecular-based applicatinos including PCR, arrays, etc.

Format Spin Column
DNA Recovery

Micro: Up to 5 μg total DNA is eluted in ≥10 μl DNA Elution Buffer per sample.

Mini: Up to 25 μg total DNA is eluted in ≥25 μl DNA Elution Buffer per sample.

Midi: Up to ≈125 μg total DNA is eluted in ≥150 μl DNA Elution Buffer per sample.

Processing Time

Micro & Mini: 15 minutes

Midi: 25 minutes

Equipment

Micro & Mini: Microcentrifuge, Vortex, Cell Disruptor/Pulverizer (optional)

Midi: Centrifuge, Vaccum Source and Manifold, Microcentrifuge, Cell Disruptor/Pulverizer w/50 ml tubes adapter

DNA Size Limits

Micro & Mini: Capable of recovering genomic DNA up to and above 40 Kb. In most instances, mitochondrial DNA and viral DNA (if present= will also be recovered.

Midi: Capable of recovering genomic DNA size fragments from 100 bp to ≥40 Kb. Typical fragments sizes range from 25 to 35 Kb. If present, parasitic and irla DNA will also be recovered.

Sample Sources

Micro & Mini: Up to 150 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.

Midi: Up to 375 mg that include leaves, stems, buds, flowers, fruit, seeds, etc.

DNA Purity

High quality, inhibitor-fee DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. A260/280 > 1.8

DNA Yield

Typically 20-80 ng DNA/ng Plant material is recovered

 

´´´´´´´´´´´´

Comparison of DNA yields from varios plant and seed samples using the ZR Plant/Seed DNA kits. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0,8% (w/v) agarose/ethidium bromide gel. M isa a 1 Kb DNA size marker (Zymo Research). Arabidopsis thaliana (1), Juniper (2), Corn Kernel (3, 4), Sunflower seed (5, 6).

 

Product Name Size Ref.:
ZR Plant/Seed DNA MicroPrep 50 Preps D6022
ZR Plant/Seed DNA MiniPrep 50 Preps D6020
ZR Plant/Seed DNA MidiPrep 25 Preps D6120
 

     Información adicional del producto
     ZR Plant/Seed DNA Kit
     Cantidad:

     Archivos Adjuntos :
Ficha Seguridad ZR Plant Micro
Ficha Seguridad ZR Plant Midi
Protocolo ZR Plant Micro
Protocolo ZR Plant Mini
Protocolo ZR Plant Midi
Ficha Técnica ZR Plant



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Nuestros distribuidores

Ptge. Dos de Maig, 9-11 . 08041 Barcelona
E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
E-mail: ecogenm@ecogen.com

 

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