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GENÓMICA

ANÁLISIS METILACIÓN

ELECTROFORESIS

EXTRACCIÓN DNA

EXTRACCION RNA

MICROARRAYS

PCR

REACTIVOS QUÍMICOS

PROTEÓMICA

DIAGNÓSTICO

INSTRUMENTACIÓN

CONSUMIBLES


 
MasterPure DNA Extraction Kit
 

MasterPure™ DNA & RNA Purification Kits use a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table 1 and Figure 1).

The gentle salt precipitation protocol (Figure 2) permits rapid purification of high molecular weight nucleic acids from a single sample or from many samples that are processed simultaneously. High yields of nucleic acids are obtained using MasterPure Kits (e.g., see Table 2), and yields and purity are highly consistent for different samples of the same material (Figure 3). The resultant nucleic acids have an A260/280 ratio of 1.8-2.0, indicating that they are free of protein. The TNA, DNA and/or RNA purified from different samples can be used directly in many applications. For example, the sensitivity of PCR or RT-PCR assays is often improved if the template is obtained using a MasterPure DNA or RNA Purification Kit (Figure 4).

Applications

  • Purify nucleic acid suitable for many applications, including PCR, RT-PCR, cloning, sequencing, Northern and Southern blotting, restriction analysis, and genomic library preparation.

Benefits

  • Extensive sample range—Purify DNA or RNA from any sample, every time (Table 1 and Figure 1).
  • Quick, easy protocol—purify TNA in 30 minutes, DNA in 45 minutes, and RNA in 60 minutes, without cumbersome columns (Figure 2).
  • High purity—O.D.260/280 ratios consistently between 1.8 and 2.0.
  • High yields (Table 2)—e.g. recover >90% of theoretical DNA yield from E. coli.
  • Consistent results (Figure 3).
  • High sensitivity—completely recover even small amounts of nucleic acid (Figure 4).
  • Safe—no phenol, chloroform, or other caustic solvents.

Reference

  1. Schanke, J. and Watson, J. (1998) EPICENTRE Forum 5 (2), 12.

Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits. Using an extremely simple salt precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.

TargetsSamples Extracted

Human & Mammalian
RNA & Genomic DNA
HIV
E. coli
HCV
B. pertussis
RSV
Yeast
M. tuberculosis
Enterovirus
HPV
Soy (tofu)
Maize
Insect tissues
Streptococcus mutans

Serum
Plasma
Whole blood
Guthrie cards
Buccal cells
Liver
Mouse tail
Kidney
Saliva
Urine
Sputum
Tissue culture cell lines
Cervical cells
Paraffin-embedded tissues





















Figure 1. DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit.
M = kb ladder.

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Figure 2. Basic MasterPure™ Complete Kit extraction protocol

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Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.

*For extraction of yeast DNA, the MasterPure Yeast DNA Purification Kit is recommended.

SampleSample SizeTNA µgDNA µgRNA µg
HeLa/HL60 cells1 X 106 cells10-303-127-15
Tissues
Liver5 mg33-425-1013-25
Brain5 mg9-136-94-11
Heart5 mg6-104-74-5
Kidney5 mg10-173-814-17
Thymus5 mg15-306-129-18
Blood200 µl3-103-90.1-0.2
Buffy coat300 µl40-5540-553-6
Mouse tail0.5 cm25-309-11aaa
E. coli3.5 x 106 cells2.5-2.81.3-1.61.6-1.8
S. mutans1.5 ml0.9aaaaaa
*Yeast2.2 x 106 cellsaaaaaa11-18
(S. cerevisiae)1.1 x 107 cellsaaaaaaa70-78




































Figure 3. Consistent purification of DNA, total nucleic acid, and RNA.
Five different genomic DNA (Lanes 1-5), total nucleic acid
(Lanes 6-10), and total cellular RNA (Lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. M = kb ladder.

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Figure 4. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin column kit. PCR amplification was performed after extraction from the indicated number of E. coli cells

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ProductCatalog No.Size
MasterPure™ Complete DNA and RNA Purification KitMC89010

5/10 Purifications*

    MC85200

200 Purifications*

For isolating TNA, DNA, or RNA.   
Contents: Red Cell Lysis Solution, Tissue and Cell Lysis Solution, MPC
Protein Precipitation Reagent, 2X T&C Lysis Solution, TE Buffer**,
RNase A, DNase I, RNase-Free, Proteinase K and 1X DNase Buffer
   

*NOTE: Cat. No. MC89010 contains reagents for 10 TNA, 10 DNA,
or 5 RNA purifications and Cat. No. MC85200 contains reagents for 200 TNA,
200 DNA, or 100 RNA purifications.

   
**Not provided with the 10 purifications kit (Cat. No. MC89010).    
MasterPure™ DNA Purification Kit (for isolating TNA or DNA)MCD85201

200 Purifications

For a small size kit, see the small MasterPure Complete Kit above, catalog #MC89010.   
Contents:
Red Cell Lysis Solution
Tissue and Cell Lysis Solution
MPC Protein Precipitation Reagent
2X T&C Lysis Solution
TE Buffer**
RNase A
Proteinase K
   
**Not provided with the 10 purifications kit (Cat. No. MC89010).   
MasterPure™ RNA Purification Kit (for isolating RNA only)MCR85102

100 Purifications

For a small size kit, see the small MasterPure Complete Kit above, catalog #MC89010.   
Contents:
Red Cell Lysis Solution
Tissue and Cell Lysis Solution
MPC Protein Precipitation Reagent
2X T&C Lysis Solution
TE Buffer**
DNase I, RNase-Free
Proteinase K
1X DNase Buffer**
   
Not provided with the 5 purifications kit (Cat. No. MC89010).   

 

     Información adicional del producto
     MasterPure Complete
     MasterPure DNA
     MasterPure RNA
     Cantidad:

     Archivos Adjuntos :
Protocolo DNA
Protocolo DNA/RNA


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E-mail: ecogen@ecogen.com


San Hermenegildo, 16 Esc. 1, 1º B .28015 Madrid
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